Pyrene, as a member of polycyclic aromatic hydrocarbons, mainly exists in the fractions of coal tar and asphalt, and has the characteristics of rich electrons, large ring conjugated, easy chemical modification, good crystallinity and so on. Pyrene fluorophore has been widely used in light-emitting diodes, bio-optoelectronic sensors and other research fields due to its high fluorescence efficiency, carrier mobility and excimer emission efficiency.
Fig.1 The chemical structure of pyrene
Fig.2 Electrochemical luminescence of pyrene derivatives 
Fig.3 Plausible mechanism of Pyr-1complexation with Ni2+ ion and removal of Ni2+ by EDTA.
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